Paula Radcliffe led the way as more than 17,000 runners filled the Olympic Stadium for the inaugural Morrisons Great Newham London Run.
For many it was their first glimpse inside the venue, which is currently being transformed, and participants soaked up the iconic scenery as they completed distances of 10k, a 2k Family Run or the Team Relay.
Thames Valley Harriers runner Fiona Thompson was the first to cross the finish line with a time of 36 minutes and 50 seconds.
She said: “The crowd were fantastic.
“They really lift you and help you find a little bit more and that’s something you wouldn’t normally get unless you were competing at the Olympics.”
Marathon world record holder Paula said: “Being in the stadium where so many dreams came true in 2012 is really special.
“I’m so glad that people are having the opportunity to use this amazing venue.”
Runners pounded the pavements of the Olympic Park on a circuit that included passing by the Copper Box, the London Aquatics Centre and the Lee Valley VeloPark before they crossed the finish line inside the stadium.
The annual event is aiming to attract 60,000 competitors over the next five years.
It rounded off a weekend of sport which saw a combined 24,000 fitness fans take part in the challenge or the Great London Newham Swim.
Entries for next year are now open .