The England rugby league team will return to the Queen Elizabeth Olympic Park for the 2016 Four Nations.
A year since their International Series win over New Zealand at the Stratford venue, the England players will go head-to-head with world champions Australia in November.
The game will be the first time the two sides have met since the 2013 World Cup (England lost 28-20), and will be the sixth match of the tournament.
England Captain Sean O’Loughlin said: “Playing at iconic venues such as the former Olympic Stadium and Anfield are one of the reasons we play the game.
“Walking out in front of 45,000 fans in London last year was one of the highlights of 2015 for me and I am sure playing in front of big home crowds will push the team on to what will hopefully be a successful campaign.”
England v Australia will be the first international rugby league match to be held at the stadium after it reopens in the summer.
It is one of six venues to be used across the country alongside stadiums in Liverpool, Coventry and Hull.
Olympic Park chief executive Linda Lennon said: “We’re delighted to be once again hosting some of the world’s best rugby league players.
“The stadium is renowned for its electric atmosphere, which will create a thrilling match for the fans and hopefully propel England to Four Nations glory.”
The game, on Friday, November 13, starts at 3.30pm with full price tickets starting at £20 .