Ticket windows in Canary Wharf Tube station will close permanently as part of station improvements.
Transport for London (TfL) said the modernisation plan and changes to the ticket hall meant staff would be moved to the latter in order to advise travellers.
The change will start on Monday, December 7.
Meanwhile, the east London station will remain staffed between the first and last train times.
A statement read: “The station now has smarter ticket machines, offering guidance in 17 languages, making paying for travel easier; staff will be on hand to show you how much more these machines can do.”
Customers are now advised to use smarter ticket machines or buy tickets and top up Oyster cards online, or using nearby Oyster Ticket Stops.
TfL also suggests using a contactless payment card.
Customers will be charged the same fare as that paid on an Oyster, without the need to continually top up.
The improvement work is expected to last for up to one month.
The station will remain open.