A series of free trials for commuters interested in travelling by coach from Folkestone, Hythe or Ashford to Greenwich will launch next month.
The trials begin on March 2 with further routes set to follow in February to include commuters from Canterbury.
Buzzlines already operates a weekday service from South Ashford direct to Canary Wharf, but the three new services will terminate at The O2.
Buzzlines chief executive Lynn Woods said: "With the Blackwall Tunnel bottleneck, it is clear the coach commute should aim to get customers on to the underground or DLR rather than adding more coaches to all the city centre congestion.
"From Ashford we can get to North Greenwich as quickly as the train gets to London Bridge. It's best to let the underground take over from there."
Commuters looking to cycle the rest of their commute can stow full-size bikes in the coach luggage hold. The Jubilee line, Emirates Airline and a number of buses run from North Greenwich.
Even allowing for travelcards, Buzzlines' new services are expected to save commuters typically between £2,000 and £3,000 a year.
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